Key features and details
- Rabbit polyclonal to LIM Kinase 1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LIM Kinase 1 antibody
See all LIM Kinase 1 primary antibodies
DescriptionRabbit polyclonal to LIM Kinase 1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human LIM Kinase 1 aa 597-647 (C terminal).
- HeLa whole cell lysate (ab150035)
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95186 was affinity purified using an epitope specific to LIMK1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab95186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
Cellular localizationCytoplasm. Cell projection > growth cone.
- Information by UniProt
- EC 188.8.131.52 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
All lanes : Anti-LIM Kinase 1 antibody (ab95186) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 30 seconds
ab95186 at 3 µg/mg precipitating LIMK1 from HeLa whole cell lysate.
Lane 1; HeLa whole cell lysate. Lane 2; Control IgG. 1mg of lysate used for IP and 20% of IP loaded.
Detection utilised ab95186 at 0.4 µg/ml followed by ECL with a 5 second exposure.
ab95186 has been referenced in 2 publications.
- Nandy SB et al. Glucose insult elicits hyperactivation of cancer stem cells through miR-424-cdc42-prdm14 signalling axis. Br J Cancer 117:1665-1675 (2017). PubMed: 29024936
- Bracamontes CG et al. The serum protein profile of early parity which induces protection against breast cancer. Oncotarget 7:82538-82553 (2016). WB ; Human . PubMed: 27769065