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Synthetic peptide within Human LIM kinase 2 aa 600-700 (C terminal). The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab45165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/2000. Predicted molecular weight: 72 kDa.|
Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LIMK2 (LIM kinase 2) knockout HAP1 whole cell lysate (40 µg)
Lane 3: Jurkat whole cell lysate (40 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab45165 was shown to specifically recognize LIMK2 (LIM kinase 2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIM kinase 2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
ab45165 staining human LIM kinase 2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"