Overview

  • Product name
  • Description
    Rabbit polyclonal to LIM1
  • Host species
    Rabbit
  • Specificity
    Does not appear to crossreact with LIM5 (the closest homologue of LIM1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation.
  • Tested applications
    Suitable for: IHC-P, ICC, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish
  • Immunogen

    C-terminal portion of frog LIM-1 protein.

  • Positive control
    • Tested with P19 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab14554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
ICC 1/500.
WB 1/3000 - 1/6000. Predicted molecular weight: 44 kDa.
IP 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificity
    Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain
    The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Images

  • ab14554 staining LIM1 in human fetal kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Actin filaments stained with phalloidin (red).

    See Abreview

  • ab14554 at 1/200 dilution staining mouse postnatal day 5 retina cells (known to express LIM 1) by ICC/IF. The cells were formaldehyde fixed and incubated with the antibody for 72 hours. An Alex-Fluor 488 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows strong horizontal cell process labeling with fainter nuclear label.

    See Abreview

References

This product has been referenced in:
  • Lui NC  et al. Lhx1/5 control dendritogenesis and spine morphogenesis of Purkinje cells via regulation of Espin. Nat Commun 8:15079 (2017). Read more (PubMed: 28516904) »
  • Peng XG  et al. Evolution of correlation between Helicobacter pylori infection and autoimmune liver disease. Exp Ther Med 14:1487-1490 (2017). Read more (PubMed: 28810614) »
See all 6 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (renal cancer (clear cell Renal Cell Carcinoma))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
renal cancer (clear cell Renal Cell Carcinoma)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 18 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (nephric duct (chiken))
Permeabilization
Yes - 0.25% triton in PBS
Specification
nephric duct (chiken)
Blocking step
(agent) for 15 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (fetal kidney cells)
Specification
fetal kidney cells
Permeabilization
Yes - 0.5% triton in pbs
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 24 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Chicken Tissue sections (E5 Spinal cord)
Specification
E5 Spinal cord
Permeabilization
Yes - triton x-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (postnatal day 5 retina)
Specification
postnatal day 5 retina
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 17 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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