Overview

  • Product name
  • Description
    Rabbit polyclonal to LIM1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human, Chimpanzee
  • Immunogen

    Recombinant fragment within Human LIM1 (internal sequence). The exact sequence is proprietary.
    Database link: P48742

  • Positive control
    • IHC-Fr: E13.5 rat brain tissue; Adult mouse retina tissue. WB: Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue; Mouse hind brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab229474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-Fr 1/100 - 1/1000.

Target

  • Function
    Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificity
    Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain
    The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Images

  • Frozen sectioned E13.5 rat brain tissue stained for LIM1 using ab229474 at 1/250 dilution in immunohistochemical analysis.

    Red: SOX2, stained by SOX2 antibody at 1/250. dilution
    Blue: Fluoroshield with DAPI.

  • Frozen sectioned adult mouse retina tissue stained for LIM1 using ab229474 at 1/250 dilution in immunohistochemical analysis.

    Red: Protein kinase C alpha staining.
    Blue: Fluorshield with DAPI.

  • All lanes : Anti-LIM1 antibody (ab229474) at 1/2000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded rat brain tissue stained for LIM1 using ab229474 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse hind brain tissue stained for LIM1 using ab229474 at 1/500 dilution in immunohistochemical analysis.

References

ab229474 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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