Overview

  • Product name

  • Description

    Rabbit polyclonal to LIM1/LHX1
  • Host species

    Rabbit
  • Specificity

    Does not appear to crossreact with LIM5 (the closest homologue of LIM1/LHX1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation.

  • Tested applications

    Suitable for: IHC-P, ICC, WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish
  • Immunogen

    Recombinant fragment corresponding to Xenopus laevis LIM1/LHX1 (C terminal).

  • Positive control

    • Tested with P19 cell line.
  • General notes

     This product was previously labelled as LIM1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab14554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000.
ICC 1/500.
WB 1/3000 - 1/6000. Predicted molecular weight: 44 kDa.
IP 1/200.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificity

    Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Images

  • ab14554 staining LIM1/LHX1 in human fetal kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Actin filaments stained with phalloidin (red).

    See Abreview

  • ab14554 at 1/200 dilution staining mouse postnatal day 5 retina cells (known to express LIM1/LHX1) by ICC/IF. The cells were formaldehyde fixed and incubated with the antibody for 72 hours. An Alex-Fluor 488 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows strong horizontal cell process labeling with fainter nuclear label.

    See Abreview

References

This product has been referenced in:

  • Lui NC  et al. Lhx1/5 control dendritogenesis and spine morphogenesis of Purkinje cells via regulation of Espin. Nat Commun 8:15079 (2017). Read more (PubMed: 28516904) »
  • Peng XG  et al. Evolution of correlation between Helicobacter pylori infection and autoimmune liver disease. Exp Ther Med 14:1487-1490 (2017). Read more (PubMed: 28810614) »
See all 6 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (renal cancer (clear cell Renal Cell Carcinoma))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
renal cancer (clear cell Renal Cell Carcinoma)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 18 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (nephric duct (chiken))
Permeabilization
Yes - 0.25% triton in PBS
Specification
nephric duct (chiken)
Blocking step
(agent) for 15 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 24 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fetal kidney cells)
Permeabilization
Yes - 0.5% triton in pbs
Specification
fetal kidney cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 24 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Chicken Tissue sections (E5 Spinal cord)
Specification
E5 Spinal cord
Permeabilization
Yes - triton x-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 27 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (postnatal day 5 retina)
Specification
postnatal day 5 retina
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 17 2006

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