Anti-LIM1/LHX1 antibody (ab229474)
Key features and details
- Rabbit polyclonal to LIM1/LHX1
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-LIM1/LHX1 antibody
See all LIM1/LHX1 primary antibodies -
Description
Rabbit polyclonal to LIM1/LHX1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Chimpanzee
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Immunogen
Recombinant fragment within Human LIM1/LHX1 (internal sequence). The exact sequence is proprietary.
Database link: P48742 -
Positive control
- IHC-Fr: E13.5 rat brain tissue; Adult mouse retina tissue. WB: Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue; Mouse hind brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
|
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| WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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| IHC-Fr | (1) |
1/100 - 1/1000.
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| Notes |
|---|
|
IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
|
IHC-Fr
1/100 - 1/1000. |
Target
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Function
Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis. -
Tissue specificity
Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Domain
The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 454600 Chimpanzee
- Entrez Gene: 786041 Cow
- Entrez Gene: 3975 Human
- Entrez Gene: 16869 Mouse
- Entrez Gene: 257634 Rat
- Omim: 601999 Human
- SwissProt: Q5IS44 Chimpanzee
- SwissProt: P48742 Human
see all -
Alternative names
- hLim-1 antibody
- Homeo box protein Lim 1 antibody
- Homeo box protein Lim1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
Paraffin-embedded rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)Frozen sectioned E13.5 rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: SOX2, stained by SOX2 antibody at 1/250. dilution
Blue: Fluoroshield with DAPI. -
Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)Frozen sectioned adult mouse retina tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: Protein kinase C alpha staining.
Blue: Fluorshield with DAPI. -
Western blot - Anti-LIM1/LHX1 antibody (ab229474)All lanes : Anti-LIM1/LHX1 antibody (ab229474) at 1/2000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)Paraffin-embedded mouse hind brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab229474 has not yet been referenced specifically in any publications.
