Anti-LIM1/LHX1 antibody (ab229474)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-LIM1/LHX1 antibody
See all LIM1/LHX1 primary antibodies -
Description
Rabbit polyclonal to LIM1/LHX1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Chimpanzee
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Immunogen
Recombinant fragment within Human LIM1/LHX1 (internal sequence). The exact sequence is proprietary.
Database link: P48742 -
Positive control
- IHC-Fr: E13.5 rat brain tissue; Adult mouse retina tissue. WB: Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue; Mouse hind brain tissue.
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General notes
This product was previously labelled as LIM1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab229474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| IHC-Fr | 1/100 - 1/1000. |
Target
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Function
Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis. -
Tissue specificity
Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Domain
The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 454600 Chimpanzee
- Entrez Gene: 786041 Cow
- Entrez Gene: 3975 Human
- Entrez Gene: 16869 Mouse
- Entrez Gene: 257634 Rat
- Omim: 601999 Human
- SwissProt: Q5IS44 Chimpanzee
- SwissProt: P48742 Human
see all -
Alternative names
- hLim-1 antibody
- Homeo box protein Lim 1 antibody
- Homeo box protein Lim1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)Paraffin-embedded rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)Frozen sectioned E13.5 rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: SOX2, stained by SOX2 antibody at 1/250. dilution
Blue: Fluoroshield with DAPI. -
Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)Frozen sectioned adult mouse retina tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.
Red: Protein kinase C alpha staining.
Blue: Fluorshield with DAPI. -
Western blot - Anti-LIM1/LHX1 antibody (ab229474)All lanes : Anti-LIM1/LHX1 antibody (ab229474) at 1/2000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)Paraffin-embedded mouse hind brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab229474 has not yet been referenced specifically in any publications.
