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    lim1lhx1-antibody-ab229474.pdf

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Anti-LIM1/LHX1 antibody (ab229474)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
  • Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)
  • Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)
  • Western blot - Anti-LIM1/LHX1 antibody (ab229474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)

Key features and details

  • Rabbit polyclonal to LIM1/LHX1
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-LIM1/LHX1 antibody
    See all LIM1/LHX1 primary antibodies
  • Description

    Rabbit polyclonal to LIM1/LHX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Human, Chimpanzee
  • Immunogen

    Recombinant fragment within Human LIM1/LHX1 (internal sequence). The exact sequence is proprietary.
    Database link: P48742

  • Positive control

    • IHC-Fr: E13.5 rat brain tissue; Adult mouse retina tissue. WB: Mouse and rat brain tissue extracts. IHC-P: Rat brain tissue; Mouse hind brain tissue.
  • General notes

     This product was previously labelled as LIM1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • LIM
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Embryogenesis
    • Axis formation and symmetry
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)

Applications

Our Abpromise guarantee covers the use of ab229474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-Fr 1/100 - 1/1000.

Target

  • Function

    Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificity

    Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P48742 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 454600 Chimpanzee
    • Entrez Gene: 786041 Cow
    • Entrez Gene: 3975 Human
    • Entrez Gene: 16869 Mouse
    • Entrez Gene: 257634 Rat
    • Omim: 601999 Human
    • SwissProt: Q5IS44 Chimpanzee
    • SwissProt: P48742 Human
    • SwissProt: P63006 Mouse
    • SwissProt: P63007 Rat
    • Unigene: 443727 Human
    • Unigene: 4965 Mouse
    • Unigene: 127825 Rat
    see all
  • Alternative names

    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)

    Paraffin-embedded rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)
    Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)

    Frozen sectioned E13.5 rat brain tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.

    Red: SOX2, stained by SOX2 antibody at 1/250. dilution
    Blue: Fluoroshield with DAPI.

  • Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)
    Immunohistochemistry (Frozen sections) - Anti-LIM1/LHX1 antibody (ab229474)

    Frozen sectioned adult mouse retina tissue stained for LIM1/LHX1 using ab229474 at 1/250 dilution in immunohistochemical analysis.

    Red: Protein kinase C alpha staining.
    Blue: Fluorshield with DAPI.

  • Western blot - Anti-LIM1/LHX1 antibody (ab229474)
    Western blot - Anti-LIM1/LHX1 antibody (ab229474)
    All lanes : Anti-LIM1/LHX1 antibody (ab229474) at 1/2000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM1/LHX1 antibody (ab229474)

    Paraffin-embedded mouse hind brain tissue stained for LIM1/LHX1 using ab229474 at 1/500 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab229474? Please let us know so that we can cite the reference in this datasheet.

    ab229474 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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