Overview

  • Product name

  • Description

    Rabbit polyclonal to LIM1/LHX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Hamster, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human LIM1/LHX1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104205)

  • Positive control

    • This antibody gave a positive signal in Human thymus tissue lysate.
  • General notes

     This product was previously labelled as LIM1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab84789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificity

    Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Domain

    The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Images

  • Anti-LIM1/LHX1 antibody (ab84789) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes

References

ab84789 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab84789.
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