Key features and details
- Rabbit polyclonal to LIM1/LHX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LIM1/LHX1 antibody
See all LIM1/LHX1 primary antibodies
DescriptionRabbit polyclonal to LIM1/LHX1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Hamster, Dog, Chimpanzee
Synthetic peptide corresponding to Human LIM1/LHX1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human thymus tissue lysate.
This product was previously labelled as LIM1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab84789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).|
FunctionPotential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
Tissue specificityExpressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
DomainThe LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
- Information by UniProt
- hLim-1 antibody
- Homeo box protein Lim 1 antibody
- Homeo box protein Lim1 antibody
Anti-LIM1/LHX1 antibody (ab84789) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
ab84789 has not yet been referenced specifically in any publications.