Recombinant Anti-LIM2 antibody [EPR16947] (ab198502)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16947] to LIM2
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-LIM2 antibody [EPR16947]
See all LIM2 primary antibodies -
Description
Rabbit monoclonal [EPR16947] to LIM2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse and Rat eyeball lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16947 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse eye tissue lysate - total protein (ab4029)
- Rat eye tissue lysate - total protein (ab4035)
- Mouse spleen tissue lysate - total protein (ab7937)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis. -
Tissue specificity
Eye lens specific. -
Involvement in disease
Cataract, multiple types 19 -
Sequence similarities
Belongs to the PMP-22/EMP/MP20 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 233187 Mouse
- Entrez Gene: 114903 Rat
- SwissProt: P56563 Mouse
- SwissProt: P54825 Rat
- Unigene: 267027 Mouse
- Unigene: 9688 Rat
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Alternative names
- Lens fiber membrane intrinsic protein antibody
- Lens intrinsic membrane protein 2 19kDa antibody
- Lens intrinsic membrane protein 2 antibody
see all
Images
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All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/50000 dilution
Lane 1 : Mouse eyeball tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/5000 dilution
Lane 1 : Rat eyeball tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab198502 has not yet been referenced specifically in any publications.