Overview

  • Product name

    Anti-LIM2 antibody [EPR16947]
    See all LIM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR16947] to LIM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LIM2 aa 50-150. The exact sequence is proprietary.
    Database link: P55344

  • Positive control

    • Mouse and Rat eyeball lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab198502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function

    Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.
  • Tissue specificity

    Eye lens specific.
  • Involvement in disease

    Cataract, multiple types 19
  • Sequence similarities

    Belongs to the PMP-22/EMP/MP20 family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Lens fiber membrane intrinsic protein antibody
    • Lens intrinsic membrane protein 2 19kDa antibody
    • Lens intrinsic membrane protein 2 antibody
    • LIM 2 antibody
    • Lim2 antibody
    • LMIP_HUMAN antibody
    • MP17 antibody
    • MP18 antibody
    • MP19 antibody
    • MP20 antibody
    • To3 antibody
    see all

Images

  • All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/50000 dilution

    Lane 1 : Mouse eyeball lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-LIM2 antibody [EPR16947] (ab198502) at 1/5000 dilution

    Lane 1 : Rat eyeball lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat heart lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

References

ab198502 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab198502.
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