Key features and details
- Rabbit polyclonal to LIMD1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LIMD1 antibody
See all LIMD1 primary antibodies
DescriptionRabbit polyclonal to LIMD1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human LIMD1 aa 50-100. The exact sequence is proprietary. (NP_055055.1)
Database link: Q9UGP4
- WB: HeLa, Jurkat and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 72 kDa.|
FunctionTranscriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
Sequence similaritiesBelongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
- Information by UniProt
- AW822033 antibody
- D9Ertd192e antibody
- LIM domain-containing protein 1 antibody
All lanes : Anti-LIMD1 antibody (ab225714) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 30 seconds
LIMD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225714 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225714 at 0.4 µg/ml.
Lane 1: ab225714 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225714 has not yet been referenced specifically in any publications.