Overview

  • Product name

  • Description

    Rabbit polyclonal to LIMD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Guinea pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human LIMD1 aa 50-100. The exact sequence is proprietary. (NP_055055.1)
    Database link: Q9UGP4

  • Positive control

    • WB: HeLa, Jurkat and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.

Target

  • Function

    Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the zyxin/ajuba family.
    Contains 3 LIM zinc-binding domains.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW822033 antibody
    • D9Ertd192e antibody
    • LIM domain-containing protein 1 antibody
    • LIM domains containing 1 antibody
    • LIMD1 antibody
    • LIMD1_HUMAN antibody
    • OTTHUMP00000164657 antibody
    • OTTHUMP00000210317 antibody
    • rCG_25194 antibody
    see all

Images

  • All lanes : Anti-LIMD1 antibody (ab225714) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 30 seconds
  • LIMD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225714 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225714 at 0.4 µg/ml.

    Lane 1: ab225714 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab225714 has not yet been referenced specifically in any publications.

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