Product nameAnti-LIMD1 antibody
See all LIMD1 primary antibodies
DescriptionRabbit polyclonal to LIMD1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human LIMD1 aa 50-100. The exact sequence is proprietary. (NP_055055.1)
Database link: Q9UGP4
- WB: HeLa, Jurkat and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 72 kDa.|
FunctionTranscriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
Sequence similaritiesBelongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
- Information by UniProt
- AW822033 antibody
- D9Ertd192e antibody
- LIM domain-containing protein 1 antibody
All lanes : Anti-LIMD1 antibody (ab225714) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 30 seconds
LIMD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225714 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225714 at 0.4 µg/ml.
Lane 1: ab225714 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab225714 has not yet been referenced specifically in any publications.