Overview

  • Product name

  • Description

    Rabbit polyclonal to LIMK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 597-647 of Human LIMK1 (NP_002305.1)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab95186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • Tissue specificity

    Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • Involvement in disease

    Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • Cellular localization

    Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.7.11.1 antibody
    • LIM domain kinase 1 antibody
    • LIM motif-containing protein kinase antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Images

  • All lanes : Anti-LIMK1 antibody (ab95186) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 73 kDa


    Exposure time: 30 seconds
  • ab95186 at 3 µg/mg precipitating LIMK1 from HeLa whole cell lysate.
    Lane 1; HeLa whole cell lysate. Lane 2; Control IgG. 1mg of lysate used for IP and 20% of IP loaded.
    Detection utilised ab95186 at 0.4 µg/ml followed by ECL with a 5 second exposure.

References

This product has been referenced in:

  • Nandy SB  et al. Glucose insult elicits hyperactivation of cancer stem cells through miR-424-cdc42-prdm14 signalling axis. Br J Cancer 117:1665-1675 (2017). Read more (PubMed: 29024936) »
  • Bracamontes CG  et al. The serum protein profile of early parity which induces protection against breast cancer. Oncotarget 7:82538-82553 (2016). WB ; Human . Read more (PubMed: 27769065) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam. Regarding the lysis/IP buffer to use with these antibodies, they have been tested with a buffer using the following formulation: IP lysis Buffer 12.5 ml 1M NaCl (250mM) 2.5 ml 1M Tris (50mM) 500ul 0.5M EDTA (5mM) 2.5ml 10% NP-40 32 ml dH20 That is not to say that RIPA will not work, however since these are cytoplasmic proteins, it would not be necessary. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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