Overview

  • Product name

    Anti-LIMK1 (phospho T508) antibody
    See all LIMK1 primary antibodies
  • Description

    Rabbit polyclonal to LIMK1 (phospho T508)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LIMK1 (phospho T508). The exact sequence is proprietary.
    Database link: P53667

  • Positive control

    • PMA-treated HeLa cell extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab194798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100 - 1/200.
IHC-P 1/50 - 1/100.

Target

  • Function

    Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
  • Tissue specificity

    Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
  • Involvement in disease

    Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
    Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
    Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
  • Cellular localization

    Cytoplasm. Cell projection > growth cone.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.7.11.1 antibody
    • LIM domain kinase 1 antibody
    • LIM motif-containing protein kinase antibody
    • LIMK antibody
    • LIMK-1 antibody
    • limk1 antibody
    • LIMK1_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling LIMK1 (phospho T508) using ab194798 at 1/50 dilution.

  • All lanes : Anti-LIMK1 (phospho T508) antibody (ab194798) at 1/500 dilution

    Lane 1 : Untreated HeLa cell extract
    Lane 2 : PMA-treated HeLa cell extract

    Predicted band size: 72 kDa

References

This product has been referenced in:

  • Yu HX  et al. MicroRNA-384 inhibits the progression of esophageal squamous cell carcinoma through blockade of the LIMK1/cofilin signaling pathway by binding to LIMK1. Biomed Pharmacother 109:751-761 (2019). Read more (PubMed: 30551528) »
  • Lu M  et al. Inhibition of p21-activated kinase 1 attenuates the cardinal features of asthma through suppressing the lymph node homing of dendritic cells. Biochem Pharmacol 154:464-473 (2018). Read more (PubMed: 29906467) »
See all 3 Publications for this product

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