Overview

  • Product name

  • Description

    Rabbit polyclonal to LIMK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human LIMK2. Derived from around the phosphorylation site of serine 283 (R-R-S(p)-L-R).
    Database link: P53671

  • Positive control

    • 3T3 cell extract, treated with PMA (125ng/ml, 30mins); 293 and COS7 cell extracts.
  • General notes

    Previously labelled as LIM kinase 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab196957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.

Target

  • Function

    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificity

    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localization

    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • LIM domain kinase 2 antibody
    • LIM kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Images

  • All lanes : Anti-LIMK2 antibody (ab196957) at 1/500 dilution

    Lane 1 : 3T3 cell extract, treated with PMA (125ng/ml, 30mins)
    Lane 2 : 3T3 cell extract, treated with PMA (125ng/ml, 30mins) with blocking peptide

    Predicted band size: 72 kDa

  • All lanes : Anti-LIMK2 antibody (ab196957) at 1/500 dilution

    Lane 1 : 293 cell extract with blocking peptide
    Lane 2 : 293 cell extract
    Lane 3 : COS7 cell extract

    Predicted band size: 72 kDa

References

ab196957 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Adult rat brain tissue lysate)
Gel Running Conditions
Reduced Denaturing (4-15% gradiated gel)
Loading amount
30 µg
Specification
Adult rat brain tissue lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C

Alexandria Slepian

Verified customer

Submitted Oct 24 2017

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