Anti-LIMK2 antibody (ab97766)

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Western blot - Anti-LIMK2 antibody (ab97766)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody (ab97766)

Key features and details

  • Rabbit polyclonal to LIMK2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-LIMK2 antibody
    See all LIMK2 primary antibodies

  • Description

    Rabbit polyclonal to LIMK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human LIMK2 aa 294-665.
    Database link: AAH13051

  • Positive control

    • Molt-4 and Raji cell lines. 293T whole cell lysate. Paraffin-embedded CA922 xenograft.

  • General notes

    Previously labelled as LIM kinase 2. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab97766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

  • Tissue specificity

    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues by ROCK1.

  • Cellular localization

    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • LIM domain kinase 2 antibody
    • LIM kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Images

  • Western blot - Anti-LIMK2 antibody (ab97766)
    Western blot - Anti-LIMK2 antibody (ab97766)
    Anti-LIMK2 antibody (ab97766) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 78 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody (ab97766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody (ab97766)
    ab97766, at 1/500, staining paraffin-embedded CA922 xenograft by Immunohistochemistry.

References (3)

Publishing research using ab97766? Please let us know so that we can cite the reference in this datasheet.

ab97766 has been referenced in 3 publications.

  • Ren T  et al. Osteosarcoma cell intrinsic PD-L2 signals promote invasion and metastasis via the RhoA-ROCK-LIMK2 and autophagy pathways. Cell Death Dis 10:261 (2019). PubMed: 30886151
  • Xiong M  et al. NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation. Theranostics 9:7156-7167 (2019). PubMed: 31695759
  • Wang S  et al. BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells. Oncotarget 8:58625-58641 (2017). PubMed: 28938584

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