Overview

  • Product name

  • Description

    Rabbit polyclonal to LIMK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human LIMK2 aa 1-252.

  • Positive control

    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lines; A549 cells
  • General notes

    Previously labelled as LIM kinase 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab97773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificity

    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localization

    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • LIM domain kinase 2 antibody
    • LIM kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Images

  • All lanes : Anti-LIMK2 antibody (ab97773) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 72 kDa



    7.5% SDS PAGE
  • ab97773, at a 1/200 dilution, staining LIMK2 in paraformaldehyde fixed A549 cells by Immunofluorescence analysis. The lower image (panel 2) was merged with DNA probe.

References

ab97773 has not yet been referenced specifically in any publications.

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