Overview

  • Product name

    Anti-LIMK2 antibody [EP969Y] - C-terminal
    See all LIMK2 primary antibodies
  • Description

    Rabbit monoclonal [EP969Y] to LIMK2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LIMK2 aa 600-700 (C terminal). The exact sequence is proprietary.
    Database link: P53671

  • Positive control

    • WB: Wild-type HAP1 cell lysate. Jurkat cell lysate. Ramos membrane lysate. IHC-P: Human ovary carcinoma tissue. ICC/IF: Ramos cells.
  • General notes

    Previously labelled as LIM kinase 2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab45165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
WB 1/2000. Predicted molecular weight: 72 kDa.

Target

  • Function

    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificity

    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localization

    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • LIM domain kinase 2 antibody
    • LIM kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: LIMK2 (LIMK2) knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Jurkat whole cell lysate (40 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab45165 was shown to specifically recognize LIMK2 (LIMK2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIMK2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • ab45165 staining LIMK2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.

  • Immunocytochemistry/ Immunofluorescence analysis of Ramos cells labeling LIMK2 with ab45165 at 1/250. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI (blue) was used as a nuclear counterstain.

     

  • Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165) at 1/2000 dilution + Ramos (Human Burkitt's lymphoma cell line) membrane lysate

    Predicted band size: 72 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analyis of mouse upper eyelid tissue, staining LIMK2 with ab45165 at 1/200 dilution. Staining was detected using DAB.

References

This product has been referenced in:

  • Hyun HW  et al. Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death. Front Cell Neurosci 10:99 (2016). Read more (PubMed: 27147971) »
  • Ko AR  et al. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 8:58 (2015). IHC-FoFr ; Rat . Read more (PubMed: 26438559) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Es freut mich Ihnen mitteilen zu können, dass wir die Konzentration des ab45165 Anti-LIM kinase 2 antibody [EP969Y] doch bestimmt haben: Für Ihr Lot beträgt sie 0.0390 mg/ml.

The concentration is 0.0390 mg/ml.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Gel Running Conditions
Reduced Denaturing (7% tris-acetate gel)
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

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Verified customer

Submitted Jul 01 2008

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