Overview

  • Product name
    Anti-LIMK2 (phospho T505) antibody
    See all LIMK2 primary antibodies
  • Description
    Rabbit polyclonal to LIMK2 (phospho T505)
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for LIMK2 only when phosphorylated at threonine 505, but may also cross react with LIMK1 phosphorylated at tyrosine 505 based on homology.
  • Tested applications
    Suitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human LIMK2 (phospho T505).

  • Positive control
    • Breast carcinoma tissue and MCF7 cell extract.
  • General notes

    Previously labelled as LIM kinase 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab38499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificity
    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localization
    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain kinase 2 antibody
    • LIM kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Images

  • ab38499 at a 1:50 dilution staining LIMK2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : untreated.

    Right image : treated with phosphopeptide.

  • All lanes : Anti-LIMK2 (phospho T505) antibody (ab38499) at 1/500 dilution

    Lane 1 : MCF7 cell extract (5-30ug of total protein) pre-incubated with phosphopeptide.
    Lane 2 : MCF7 cell extract (5-30ug of total protein).

    Secondary
    All lanes : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)

    Predicted band size: 72 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab38499 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Xia D  et al. miR302a inhibits the proliferation of esophageal cancer cells through the MAPK and PI3K/Akt signaling pathways. Oncol Lett 15:3937-3943 (2018). Read more (PubMed: 29456742) »
  • Wang S  et al. BMPR2 promotes invasion and metastasis via the RhoA-ROCK-LIMK2 pathway in human osteosarcoma cells. Oncotarget 8:58625-58641 (2017). Read more (PubMed: 28938584) »
See all 8 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Gel Running Conditions
Reduced Denaturing (7% tris-acetate gel)
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up