Overview

  • Product name
  • Description
    Rabbit polyclonal to LIMKAIN B1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1625-1675 of Human LIMKAIN B1 (NP_055462.2).

  • Positive control
    • HeLa, Jurkat, NIH 3T3 and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156699 was affinity purified using an epitope specific to LIMKAIN B1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 193 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-LIMKAIN B1 antibody (ab156699) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 193 kDa


    Exposure time: 30 seconds
  • Lane 1 : Alternative Anti-LIMKAIN B1 antibody
    Lane 2 : Anti-LIMKAIN B1 antibody (ab156699) at 1 µg/ml
    Lane 3 : Control IgG

    All lanes : Jurkat whole cell lysate

References

ab156699 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Cow Cell lysate - whole cell (Bovine oocyte and Testis)
Specification
Bovine oocyte and Testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Mahdi Mahdipour

Verified customer

Submitted Dec 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up