Key features and details
- Rabbit polyclonal to LIMKAIN B1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LIMKAIN B1 antibody
DescriptionRabbit polyclonal to LIMKAIN B1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Guinea pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla
- HeLa, Jurkat, NIH 3T3 and 293T whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab156699 was affinity purified using an epitope specific to LIMKAIN B1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab156699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 193 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- KIAA0430 antibody
- Limkain-b1 antibody
- LKAP antibody
All lanes : Anti-LIMKAIN B1 antibody (ab156699) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 193 kDa
Exposure time: 30 seconds
Detection of LIMKAIN B1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab156699 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab156699 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1 : Alternative Anti-LIMKAIN B1 antibody
Lane 2 : Anti-LIMKAIN B1 antibody (ab156699) at 1 µg/ml
Lane 3 : Control IgG
All lanes : Jurkat whole cell lysate
ab156699 has not yet been referenced specifically in any publications.