Overview

  • Product name
    Limonene Mounting Medium (30ml)
  • General notes

    Organic mounting medium ab104141 compatible with chromogens such as DAB and alkaline phosphatase chromogens. Suitable for hematoxylin and eosin (H and E) staining.

    This mounting medium is made with limonene, a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. This mounting medium is also suitable with alkaline phosphatase chromogens. It is an excellent choice for mounting H and E stained slides.

    Intended Use Mounting tissues, cell smears in Immunohistochemistry for chromogens that are resistant to organic solvents e.g. DAB, DAB with cobalt, DAB with Nickel any other chromogens that are resistant to organic solvents dehydration. H & E (hematoxylin and Eosin) staining

    Other products for IHC

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Procedure

    • Dehydrate tissue or cell smear slides with dehydrating agents e.g. 30 to 100% alcohol followed by xylene, toluene or any other dehydrating reagents.
    • Add 2-3 drops of mounting medium. Apply coverslip carefully, avoiding air bubbles.
    • Visualize slide under a microscope. The slides can be dried by leaving at room temperature overnight or by heating at 37-40°C for several hours.

Properties

References

ab104141 has not yet been referenced specifically in any publications.

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