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    limonene-mounting-medium-ab104141.pdf

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Limonene Mounting Medium (ab104141)

  • Datasheet
  • SDS
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Overview

  • Product name

    Limonene Mounting Medium
  • General notes

    Limonene Mounting Medium ab104141 is an organic mounting medium compatible with chromogens such as DAB. Suitable for hematoxylin and eosin (H and E) staining.

    This mounting medium is made with limonene, a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. It is an excellent choice for mounting H and E stained slides.

    Intended Use Mounting tissues, cell smears in Immunohistochemistry for chromogens that are resistant to organic solvents e.g. DAB, DAB with cobalt, DAB with Nickel any other chromogens that are resistant to organic solvents dehydration. H & E (hematoxylin and Eosin) staining.

    Mounting media products

    For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium ab104135, Mounting Medium with DAPI ab104139, and Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

    For chromogenic immunohistochemistry with the organic solvent-soluble substrates AEC or Fast Red, we recommend aqueous Mounting Medium ab64230.

     

    Procedure

    • Dehydrate tissue or cell smear slides with dehydrating agents e.g. 30 to 100% alcohol followed by xylene, toluene or any other dehydrating reagents.
    • Add 2-3 drops of mounting medium. Apply coverslip carefully, avoiding air bubbles.
    • Visualize slide under a microscope. The slides can be dried by leaving at room temperature overnight or by heating at 37-40°C for several hours.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at room temperature.
  • Storage buffer

    Constituent: 75% D-Limonene
  • Concentration information loading...
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    • Mounting Medium

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab104141? Please let us know so that we can cite the reference in this datasheet.

ab104141 has been referenced in 1 publication.

  • Vázquez-Arreguín K  et al. Oct1/Pou2f1 is selectively required for colon regeneration and regulates colon malignancy. PLoS Genet 15:e1007687 (2019). PubMed: 31059499

Customer reviews and Q&As

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EXCELLENT Mounting Medium

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
EXCELLENT Mounting Medium
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 29 2020

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