Overview

  • Product name

  • Description

    Goat polyclonal to LIMPII
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-NKANIQFGDNGTTIS

    , corresponding to internal sequence amino acids 96-110 of human LIMPII (NP_005497.1).

  • Positive control

    • Human brain (cortex and cerebellum) lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab87637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • Involvement in disease

    Epilepsy, progressive myoclonic 4, with or without renal failure
    Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
  • Sequence similarities

    Belongs to the CD36 family.
  • Cellular localization

    Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 85 kDa lysosomal membrane sialoglycoprotein antibody
    • 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
    • AMRF antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II) antibody
    • CD36 antigen antibody
    • CD36 antigen-like 2 antibody
    • CD36L2 antibody
    • EPM4 antibody
    • HLGP85 antibody
    • LGP85 antibody
    • LIMP 2 antibody
    • LIMP II antibody
    • LIMP2 antibody
    • LIMPII antibody
    • Lysosomal integral membrane protein II antibody
    • Lysosome membrane protein 2 antibody
    • Lysosome membrane protein II antibody
    • OTTHUMP00000160590 antibody
    • OTTHUMP00000219176 antibody
    • Scarb2 antibody
    • Scavenger receptor class B member 2 antibody
    • Scavenger receptor class B, member 2 antibody
    • SCRB2_HUMAN antibody
    • SR BII antibody
    • SRBII antibody
    see all

Images

  • Anti-LIMPII antibody (ab87637) at 0.1 µg/ml + human cerebral cortex lysate (in RIPA buffer) at 35 µg

    Predicted band size: 54 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • ab87637 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows lysosomal staining. Tissue pretreated using steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.

References

ab87637 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain bearing human tumor)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.2%
Specification
Brain bearing human tumor
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Oct 26 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87)
Permeabilization
Yes - TRITON 0.2%
Specification
U87
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Oct 25 2015

Answer

Thank you for contacting us. The antibody ab87637 has not been tested in either cytochemistry or histochemistry. I assume you want to stain cell cultures or tissue sections, followed by IF detection. The antibody has not been validated on either sample type for staining, only for western blotting. Our guarantee does not apply to untested applications, but the antibody may still work in this untested application. I suggest testing at least two fixatives: paraformaldehyde followed by detergent permabilization, or acetone, or methanol. After blocking non-specific interactions with serum of BSA, test a range of dilutions to determine whh gives the most signal with the least background staining. A good range to test would be 1/100 - 1/1000. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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