Key features and details
- Goat polyclonal to LIMPII
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LIMPII antibody
See all LIMPII primary antibodies
DescriptionGoat polyclonal to LIMPII
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human brain (cortex and cerebellum) lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab87637 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab87637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as a lysosomal receptor for glucosylceramidase (GBA) targeting.
(Microbial infection) Acts as a receptor for enterovirus 71.
Involvement in diseaseEpilepsy, progressive myoclonic 4, with or without renal failure
Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
Sequence similaritiesBelongs to the CD36 family.
Cellular localizationLysosome membrane.
- Information by UniProt
- 85 kDa lysosomal membrane sialoglycoprotein antibody
- 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
- AMRF antibody
Anti-LIMPII antibody (ab87637) at 0.1 µg/ml + human cerebral cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 54 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab87637 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows lysosomal staining. Tissue pretreated using steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.
ab87637 has not yet been referenced specifically in any publications.