RecombinantRabMAb

Recombinant Anti-LIMPII antibody [EPR12080] (ab176317)

Submit a review Submit a question References (5)
Western blot - Anti-LIMPII antibody [EPR12080] (ab176317)
  • Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (ab176317)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)
  • Flow Cytometry - Anti-LIMPII antibody [EPR12080] (ab176317)
  • Anti-LIMPII antibody [EPR12080] (ab176317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12080] to LIMPII
  • Suitable for: WB, Flow Cyt, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-LIMPII antibody [EPR12080]
    See all LIMPII primary antibodies

  • Description

    Rabbit monoclonal [EPR12080] to LIMPII

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human LIMPII aa 50-150. The exact sequence is proprietary.
    Database link: Q14108

  • Positive control

    • WB: HeLa and Y79 cell lysate. Rat eyeball and muscle lysate. Mouse liver lysate. IP: Y79 cell lysate. Flow Cyt: Permeabilized HeLa cells. IHC-P:Human kidney and liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab176317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
      (Microbial infection) Acts as a receptor for enterovirus 71.

    • Involvement in disease

      Epilepsy, progressive myoclonic 4, with or without renal failure
      Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.

    • Sequence similarities

      Belongs to the CD36 family.

    • Cellular localization

      Lysosome membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 85 kDa lysosomal membrane sialoglycoprotein antibody
      • 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
      • AMRF antibody
      • CD36 antibody
      • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II) antibody
      • CD36 antigen antibody
      • CD36 antigen-like 2 antibody
      • CD36L2 antibody
      • EPM4 antibody
      • HLGP85 antibody
      • LGP85 antibody
      • LIMP 2 antibody
      • LIMP II antibody
      • LIMP2 antibody
      • LIMPII antibody
      • Lysosomal integral membrane protein II antibody
      • Lysosome membrane protein 2 antibody
      • Lysosome membrane protein II antibody
      • OTTHUMP00000160590 antibody
      • OTTHUMP00000219176 antibody
      • Scarb2 antibody
      • Scavenger receptor class B member 2 antibody
      • Scavenger receptor class B, member 2 antibody
      • SCRB2_HUMAN antibody
      • SR BII antibody
      • SRBII antibody
      see all

    Images

    • Western blot - Anti-LIMPII antibody [EPR12080] (ab176317)
      Western blot - Anti-LIMPII antibody [EPR12080] (ab176317)
      All lanes : Anti-LIMPII antibody [EPR12080] (ab176317) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
      Lane 2 : Y79 (Human retinoblastoma cell line) lysate
      Lane 3 : Rat eyeball lysate
      Lane 4 : Rat muscle lysate
      Lane 5 : Mouse liver lysate

      Predicted band size: 54 kDa

    • Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (ab176317)
      Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (ab176317)

      Western blot analysis on immunoprecipitation pellet from Y79 (Human retinoblastoma cell line) cell lysate labeling LIMPII using ab176317 at a 1/10 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labling LIMPII using ab176317 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)

      Immunohistochemical analysis of paraffin-embedded human liver tissue labling LIMPII using ab176317 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-LIMPII antibody [EPR12080] (ab176317)
      Flow Cytometry - Anti-LIMPII antibody [EPR12080] (ab176317)

      Flow cytometric analysis of permeabilized HeLa cells labeling LIMPII using ab176317 at a 1/10 dilution (red) or a rabbit IgG control (green).

    • Anti-LIMPII antibody [EPR12080] (ab176317)
      Anti-LIMPII antibody [EPR12080] (ab176317)

    References (5)

    Publishing research using ab176317? Please let us know so that we can cite the reference in this datasheet.

    ab176317 has been referenced in 5 publications.

    • Eriksson I  et al. Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins. Cell Death Dis 11:370 (2020). PubMed: 32409651
    • Birket MJ  et al. A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. Stem Cell Reports 13:380-393 (2019). PubMed: 31378672
    • Conrad KS  et al. Lysosomal integral membrane protein-2 as a phospholipid receptor revealed by biophysical and cellular studies. Nat Commun 8:1908 (2017). PubMed: 29199275
    • Aizawa S  et al. Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes. Autophagy 13:218-222 (2017). WB ; Human . PubMed: 27846365
    • Aizawa S  et al. Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes. Autophagy 12:565-78 (2016). PubMed: 27046251

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