Recombinant Anti-LIMPII antibody [EPR12080] (ab176317)
- Datasheet
- References (3)
- Protocols
Overview
-
Product name
Anti-LIMPII antibody [EPR12080]
See all LIMPII primary antibodies -
Description
Rabbit monoclonal [EPR12080] to LIMPII -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IP, IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LIMPII aa 50-150. The exact sequence is proprietary.
Database link: Q14108 -
Positive control
- HeLa, Y79, Rat eyeball, Rat muscle and Mouse liver lysates, Human liver and kidney tissues
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12080 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab176317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 54 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
(Microbial infection) Acts as a receptor for enterovirus 71. -
Involvement in disease
Epilepsy, progressive myoclonic 4, with or without renal failure
Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease. -
Sequence similarities
Belongs to the CD36 family. -
Cellular localization
Lysosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 950 Human
- Entrez Gene: 12492 Mouse
- Entrez Gene: 117106 Rat
- Omim: 602257 Human
- SwissProt: Q14108 Human
- SwissProt: O35114 Mouse
- SwissProt: P27615 Rat
- Unigene: 349656 Human
see all -
Alternative names
- 85 kDa lysosomal membrane sialoglycoprotein antibody
- 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
- AMRF antibody
see all
Images
-
![Western blot - Anti-LIMPII antibody [EPR12080] (ab176317)](https://www.abcam.com/ps/products/176/ab176317/Images/ab176317-203510-anti-limpii-antibody-epr12080-western-blot.jpg)
Western blot - Anti-LIMPII antibody [EPR12080] (ab176317)All lanes : Anti-LIMPII antibody [EPR12080] (ab176317) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : Y79 lysate
Lane 3 : Rat eyeball lysate
Lane 4 : Rat muscle lysate
Lane 5 : Mouse liver lysate
Predicted band size: 54 kDa -
![Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (ab176317)](https://www.abcam.com/ps/products/176/ab176317/Images/ab176317-203514-anti-limpii-antibody-epr12080-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LIMPII antibody [EPR12080] (ab176317)Western blot analysis on immunoprecipitation pellet from Y79 cell lysate labeling LIMPII using ab176317 at a 1/10 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)](https://www.abcam.com/ps/products/176/ab176317/Images/ab176317-203512-anti-limpii-antibody-epr12080-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)Immunohistochemical analysis of paraffin-embedded kidney tissue labling LIMPII using ab176317 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)](https://www.abcam.com/ps/products/176/ab176317/Images/ab176317-203511-anti-limpii-antibody-epr12080-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMPII antibody [EPR12080] (ab176317)Immunohistochemical analysis of paraffin-embedded Human liver tissue labling LIMPII using ab176317 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Flow Cytometry - Anti-LIMPII antibody [EPR12080] (ab176317)](https://www.abcam.com/ps/products/176/ab176317/Images/ab176317-203513-anti-limpii-antibody-epr12080-flow-cytometry.jpg)
Flow Cytometry - Anti-LIMPII antibody [EPR12080] (ab176317)Flow cytometric analysis of permeabilized HeLa cells labeling LIMPII using ab176317 at a 1/10 dilution (red) or a rabbit IgG control (green).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Aizawa S et al. Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes. Autophagy 13:218-222 (2017). WB ; Human . Read more (PubMed: 27846365) »
- Conrad KS et al. Lysosomal integral membrane protein-2 as a phospholipid receptor revealed by biophysical and cellular studies. Nat Commun 8:1908 (2017). Read more (PubMed: 29199275) »
