Key features and details
- Rabbit polyclonal to Lin28
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Lin28 antibody
See all Lin28 primary antibodies
DescriptionRabbit polyclonal to Lin28
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
- Mouse placenta and embryo tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab218028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
Sequence similaritiesBelongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
DomainThe CSD domain is required for function in muscle differentiation.
Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
- Information by UniProt
- AL024421 antibody
- CSDD1 antibody
- CSDD2 antibody
Anti-Lin28 antibody (ab218028) at 1/1000 dilution + Mouse placenta lysates
Conjugated secondary antibody at 1/20000 dilution
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse placenta tissue labeling Lin28 with ab218028 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse embryo tissue labeling Lin28 with ab218028 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab218028 has not yet been referenced specifically in any publications.