Overview

  • Product name

  • Description

    Rabbit polyclonal to Lin28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Lin28 aa 90-120 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    GEAVEFTFKKSAKGLESIRVTGPGGVFCIGS


    Database link: Q9H9Z2

  • Positive control

    • Mouse placenta and embryo tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab218028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/1000.

Target

  • Function

    Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificity

    Expressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similarities

    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stage

    Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • Domain

    The CSD domain is required for function in muscle differentiation.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • AL024421 antibody
    • CSDD1 antibody
    • CSDD2 antibody
    • FLJ12457 antibody
    • Lin 28 antibody
    • Lin 28 homolog (C. elegans) antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin 28 homolog A antibody
    • Lin 28 homolog antibody
    • Lin-28A antibody
    • Lin28 antibody
    • Lin28, C. elegans, homolog of, A antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • Protein lin-28 homolog B antibody
    • RNA binding protein lin 28 antibody
    • Tex17 antibody
    • ZCCHC1 antibody
    • Zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain containing protein 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Images

  • Anti-Lin28 antibody (ab218028) at 1/1000 dilution + Mouse placenta lysates

    Secondary
    Conjugated secondary antibody at 1/20000 dilution
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse placenta tissue labeling Lin28 with ab218028 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse embryo tissue labeling Lin28 with ab218028 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab218028 has not yet been referenced specifically in any publications.

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