Key features and details
- Rabbit polyclonal to Lin28
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab46020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
Sequence similaritiesBelongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain.
Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
DomainThe CSD domain is required for function in muscle differentiation.
Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
- Information by UniProt
- AL024421 antibody
- CSDD1 antibody
- CSDD2 antibody
All lanes : Anti-Lin28 antibody (ab46020) at 1/1000 dilution
Lane 1 : PC-3 cell extract in lysis buffer containing NP-40 at 45 µg
Lane 2 : PC-3 cell extract (Trizol bottom phase) at 30 µg
Lane 3 : Tera-2 cell extract (Trizol bottom phase) at 30 µg
Lane 4 : Tera-2 cell extract in lysis buffer containing NP-40 at 45 µg
All lanes : Goat anti-rabbit HRP conjugated at 1/4000 dilution
Predicted band size: 23 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
It is unclear why lin28 runs at a larger size than its predicted moleular weight but recombinant lin28 protein is also known to run at around 30 kDa.
Human Embryonic stem cells were fixed in PFA and then incubated with the primary antibody ab46020 (1:1000) diluted in 2% BSA for 16 hours at 4C. Cells where then washed and incubated with a Cy3 conjugated goat anti-rabbit polyclonal antibody (1:400). Cells where then washed and visualised.
ab46020 has been referenced in 67 publications.
- Wu H et al. MSC-induced lncRNA HCP5 drove fatty acid oxidation through miR-3619-5p/AMPK/PGC1a/CEBPB axis to promote stemness and chemo-resistance of gastric cancer. Cell Death Dis 11:233 (2020). PubMed: 32300102
- Xing F et al. Cloning and expression of lin-28 homolog B gene in the onset of puberty in Duolang sheep. Asian-Australas J Anim Sci 32:23-30 (2019). PubMed: 30381750
- Dong L et al. Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys. Front Physiol 10:1155 (2019). PubMed: 31607938
- Yang Y et al. MiR-4319 hinders YAP expression to restrain non-small cell lung cancer growth through regulation of LIN28-mediated RFX5 stability. Biomed Pharmacother 115:108956 (2019). PubMed: 31096145
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452