Anti-Lin28A antibody (ab155542)
Key features and details
- Rabbit polyclonal to Lin28A
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Lin28A antibody
See all Lin28A primary antibodies -
Description
Rabbit polyclonal to Lin28A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-180 of Human Lin28A (Uniprot ID Q9H9Z2).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| Flow Cyt | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. -
Tissue specificity
Expressed in embryonic stem cells (ES cells), placenta and testis. -
Sequence similarities
Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain. -
Developmental stage
Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. -
Domain
The CSD domain is required for function in muscle differentiation. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. - Information by UniProt
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Database links
- Entrez Gene: 614997 Cow
- Entrez Gene: 79727 Human
- Entrez Gene: 83557 Mouse
- Omim: 611043 Human
- SwissProt: Q9H9Z2 Human
- SwissProt: Q8K3Y3 Mouse
- Unigene: 86154 Human
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Alternative names
- CSDD1 antibody
- FLJ12457 antibody
- LIN 28 antibody
see all
Images
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Western blot - Anti-Lin28A antibody (ab155542)Anti-Lin28A antibody (ab155542) at 1/3000 dilution + Human ESC whole cell lysate at 20 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody (ab155542)Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, labeling Lin28A with ab155542 at 1/500 dilution. -
Western blot - Anti-Lin28A antibody (ab155542)Anti-Lin28A antibody (ab155542) at 1/3000 dilution + Mouse ESC whole cell lysate at 20 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Flow Cytometry - Anti-Lin28A antibody (ab155542)Flow cytometry on Human embryonic stem cells, labeling Lin28A with ab155542 1/100 dilution (purple) or Rabbit IgG (Black)
Protocols
References (1)
ab155542 has been referenced in 1 publication.
- Li T et al. H. pylori infection induced BMAL1 expression and rhythm disorder aggravate gastric inflammation. EBioMedicine 39:301-314 (2019). PubMed: 30502053
