Anti-Lin28A antibody (ab155542)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Lin28A antibody
See all Lin28A primary antibodies -
DescriptionRabbit polyclonal to Lin28A
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-180 of Human Lin28A (Uniprot ID Q9H9Z2).
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Positive control
- Human ESC and Mouse ESC whole cell lysates; Human embryonic stem cells; Human gastric cancer tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/1000. | |
| Flow Cyt | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
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Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
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Sequence similaritiesBelongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain. -
Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
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DomainThe CSD domain is required for function in muscle differentiation.
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Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
- Information by UniProt
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Database links
- Entrez Gene: 614997 Cow
- Entrez Gene: 79727 Human
- Entrez Gene: 83557 Mouse
- Omim: 611043 Human
- SwissProt: Q9H9Z2 Human
- SwissProt: Q8K3Y3 Mouse
- Unigene: 86154 Human
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Alternative names
- CSDD1 antibody
- FLJ12457 antibody
- LIN 28 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lin28A antibody (ab155542)Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, labeling Lin28A with ab155542 at 1/500 dilution. -
Western blot - Anti-Lin28A antibody (ab155542)Anti-Lin28A antibody (ab155542) at 1/3000 dilution + Human ESC whole cell lysate at 20 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Western blot - Anti-Lin28A antibody (ab155542)Anti-Lin28A antibody (ab155542) at 1/3000 dilution + Mouse ESC whole cell lysate at 20 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Flow Cytometry - Anti-Lin28A antibody (ab155542)Flow cytometry on Human embryonic stem cells, labeling Lin28A with ab155542 1/100 dilution (purple) or Rabbit IgG (Black)
Protocols
References
ab155542 has not yet been referenced specifically in any publications.
