Overview

  • Product name
  • Description
    Rabbit polyclonal to Lin28A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-180 of Human Lin28A (Uniprot ID Q9H9Z2).

  • Positive control
    • Human ESC and Mouse ESC whole cell lysates; Human embryonic stem cells; Human gastric cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000.
Flow Cyt 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/1000.

Target

  • Function
    Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificity
    Expressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similarities
    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stage
    Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • Domain
    The CSD domain is required for function in muscle differentiation.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSDD1 antibody
    • FLJ12457 antibody
    • LIN 28 antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin-28A antibody
    • LIN28 antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • ZCCHC1 antibody
    • zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, labeling Lin28A with ab155542 at 1/500 dilution.
  • Anti-Lin28A antibody (ab155542) at 1/3000 dilution + Human ESC whole cell lysate at 20 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Anti-Lin28A antibody (ab155542) at 1/3000 dilution + Mouse ESC whole cell lysate at 20 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Flow cytometry on Human embryonic stem cells, labeling Lin28A with ab155542 1/100 dilution (purple) or Rabbit IgG (Black)

References

ab155542 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (GASTRIC CANCER CELL)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
30 µg
Specification
GASTRIC CANCER CELL
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1µg/mL · Temperature: 24°C
Username

Mr. JUNGHO YOON

Verified customer

Submitted Aug 16 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
Sample
Human Tissue sections (pplacenta)
Specification
pplacenta
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 01 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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