Recombinant
RabMAb

Recombinant Anti-Lin28A antibody [EPR4640] - BSA and Azide free (ab226089)

Overview

  • Product name

    Anti-Lin28A antibody [EPR4640] - BSA and Azide free
    See all Lin28A primary antibodies
  • Description

    Rabbit monoclonal [EPR4640] to Lin28A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lin28A. The exact sequence is proprietary.

  • General notes

    ab226089 is the carrier-free version of ab124765 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab226089 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse and Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4640
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

(Heat up to 98°C, below boiling, and then let cool for 10-20 min).

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificity

    Expressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similarities

    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stage

    Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • Domain

    The CSD domain is required for function in muscle differentiation.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSDD1 antibody
    • FLJ12457 antibody
    • LIN 28 antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin-28A antibody
    • LIN28 antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • ZCCHC1 antibody
    • zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Lin28A with purified ab124765 at 1/50 dilution (12 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/500 dilution (1.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Lin28A with purified ab124765 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • ab124765 (purified) at 1/30 dilution (2ug) immunoprecipitating Lin28A in NCCIT whole cell lysate. NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab124765 & NCCIT whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124765 in NCCIT whole cell lysate

    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • ab124765 (unpurified), at 1/100 dilution, staining Lin28A in paraffin-embedded Human seminoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab124765 (unpurified), at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

  • ab124765 (unpurified) showing positive staining in Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab124765 (unpurified) showing positive staining in Dysgerminoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab124765 (unpurified) showing positive staining in Embryonal carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ab124765 (unpurified) showing negative staining in Normal brain tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124765).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab226089 has not yet been referenced specifically in any publications.

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