Overview

  • Product name

    Anti-Lin28B antibody [EPR18707]
    See all Lin28B primary antibodies
  • Description

    Rabbit monoclonal [EPR18707] to Lin28B
  • Host species

    Rabbit
  • Specificity

    Note that this product recognizes recombinant Lin28B and Lin28B present in cell lysates; however, this product failed to detect Lin28B in tissue lysates in our assay conditions. Please see ab191881, which detected Lin28B in tissue lysates.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lin28B aa 100-200. The exact sequence is proprietary.
    Database link: Q6ZN17

  • Positive control

    • WB: K562, HepG2 and JAR whole cell lysates; human Lin28B full length recombinant protein. IP: JAR whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18707
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).
IP 1/50.

Target

  • Function

    Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
  • Tissue specificity

    High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level).
  • Sequence similarities

    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.
  • Information by UniProt
  • Database links

  • Alternative names

    • CSDD 2 antibody
    • CSDD2 antibody
    • FLJ16517 antibody
    • Lin 28 homolog B (C. elegans) antibody
    • Lin 28.2 antibody
    • Lin-28B antibody
    • LIN28B antibody
    • LN28B_HUMAN antibody
    • Protein lin-28 homolog B antibody
    see all

Images

  • All lanes : Anti-Lin28B antibody [EPR18707] (ab191882) at 1/2000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : JAR (Human placenta choriocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1 and 2: 3 minutes; Lane 3: 10 seconds.

  • Anti-Lin28B antibody [EPR18707] (ab191882) at 1/1000 dilution + Human Lin28B full length recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human Lin28B full length recombinant protein contains aa1-250 with a His-Tag®. This  recombinant protein was made in-house.

  • Lin28B was immunoprecipitated from 1 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab191882 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab191882 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: JAR whole cell lysate 10µg (Input).

    Lane 2: ab191882 IP in JAR whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191882 in JAR whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Lin28B was immunoprecipitated from 1 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab191882 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab191882 at 1/1000 dilution (A), or ab191881 at 1/1000 dilution (B).

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: JAR whole cell lysate 10µg (Input).

    Lane 2: ab191882 IP in JAR whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191882 in JAR whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

ab191882 has not yet been referenced specifically in any publications.

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