Anti-LIN7 antibody (ab127049)
Key features and details
- Rabbit polyclonal to LIN7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIN7 antibody -
Description
Rabbit polyclonal to LIN7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-187 of Human LIN7 (UniProt O14910).
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Positive control
- NT2D1, IMR32, U87-MG and MCF7 whole cell lysate, MCF7 cells, Human colon carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. -
Sequence similarities
Belongs to the lin-7 family.
Contains 1 L27 domain.
Contains 1 PDZ (DHR) domain. -
Domain
The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.
The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.
The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes. -
Cellular localization
Cell membrane. Basolateral cell membrane. Cell junction. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > tight junction. Cell junction > synapse > synaptosome. Enriched in synaptosomes and at epithelial cell-cell junctions (By similarity). Mainly basolateral in renal epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 55327 Human
- Entrez Gene: 64130 Human
- Entrez Gene: 8825 Human
- Entrez Gene: 108030 Mouse
- Entrez Gene: 22342 Mouse
- Entrez Gene: 22343 Mouse
- Entrez Gene: 60377 Rat
- Entrez Gene: 60442 Rat
see all -
Alternative names
- hLin 7 antibody
- hLin7 antibody
- LIN 7 antibody
see all
Images
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Anti-LIN7 antibody (ab127049) at 1/5000 dilution + MCF7 whole cell
lysate at 30 µg
Predicted band size: 22 kDa
12% SDS PAGE -
ab127049, at a dilution of 1/500, staining LIN7 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image has been costained with Hoechst 33342.
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ab127049, at a dilution of 1/250, staining LIN7 in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab127049 has not yet been referenced specifically in any publications.