Overview

  • Product name

    Anti-LIN7 antibody
  • Description

    Rabbit polyclonal to LIN7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-187 of Human LIN7 (UniProt O14910).

  • Positive control

    • NT2D1, IMR32, U87-MG and MCF7 whole cell lysate, MCF7 cells, Human colon carcinoma tissue
  • General notes

    Keep as a concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab127049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.
  • Sequence similarities

    Belongs to the lin-7 family.
    Contains 1 L27 domain.
    Contains 1 PDZ (DHR) domain.
  • Domain

    The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.
    The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.
    The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes.
  • Cellular localization

    Cell membrane. Basolateral cell membrane. Cell junction. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > tight junction. Cell junction > synapse > synaptosome. Enriched in synaptosomes and at epithelial cell-cell junctions (By similarity). Mainly basolateral in renal epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • hLin 7 antibody
    • hLin7 antibody
    • LIN 7 antibody
    • Lin 7 homolog A antibody
    • LIN 7A antibody
    • LIN 7B antibody
    • LIN 7C antibody
    • Lin-7C antibody
    • Lin7 homolog A antibody
    • LIN7A antibody
    • LIN7C antibody
    • LIN7C_HUMAN antibody
    • MALS 1 antibody
    • MALS antibody
    • MALS-3 antibody
    • MALS1 antibody
    • Mammalian LIN 7 1 antibody
    • Mammalian lin seven protein 1 antibody
    • Mammalian lin-seven protein 3 antibody
    • Mammalian LIN7 1 antibody
    • MGC148143 antibody
    • Protein lin 7 homolog B antibody
    • Protein lin 7 homolog C antibody
    • Protein lin-7 homolog C antibody
    • protein lin7 homolog A antibody
    • Tax interaction protein 33 antibody
    • TIP 33 antibody
    • TIP33 antibody
    • VELI 1 antibody
    • Veli 1 protein antibody
    • Veli-3 antibody
    • VELI1 antibody
    • Velis antibody
    • Vertebrate LIN 7 homolog 1 antibody
    • Vertebrate lin-7 homolog 3 antibody
    • Vertebrate LIN7 homolog 1 antibody
    see all

Images

  • Anti-LIN7 antibody (ab127049) at 1/5000 dilution + MCF7 whole cell
    lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS PAGE
  • ab127049, at a dilution of 1/500, staining LIN7 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image has been costained with Hoechst 33342.
  • ab127049, at a dilution of 1/250, staining LIN7 in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

References

ab127049 has not yet been referenced specifically in any publications.

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