Product nameAnti-LIN7A antibody [EPR12609]
See all LIN7A primary antibodies
DescriptionRabbit monoclonal [EPR12609] to LIN7A
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LIN7A aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O14910
- Rat brain, human fetal brain and mouse brain lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 26 kDa.|
|IP||1/10 - 1/100.|
FunctionPlays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.
Tissue specificityExpressed in brain, testis, kidney, placenta and liver.
Sequence similaritiesBelongs to the lin-7 family.
Contains 1 L27 domain.
Contains 1 PDZ (DHR) domain.
DomainThe kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.
The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.
The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes.
Cellular localizationCell membrane. Basolateral cell membrane. Cell junction. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > tight junction. Cell junction > synapse > synaptosome. Enriched in synaptosomes and at epithelial cell-cell junctions (By similarity). Mainly basolateral in renal epithelial cells.
- Information by UniProt
- hLin-7 antibody
- Lin 7 homolog A (C. elegans) antibody
- LIN 7A antibody
All lanes : Anti-LIN7A antibody [EPR12609] (ab174297) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 26 kDa
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab174297 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.