Overview

  • Product name
    Anti-LIN7A antibody [EPR12609]
    See all LIN7A primary antibodies
  • Description
    Rabbit monoclonal [EPR12609] to LIN7A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LIN7A aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O14910

  • Positive control
    • Rat brain, human fetal brain and mouse brain lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.
    • Tissue specificity
      Expressed in brain, testis, kidney, placenta and liver.
    • Sequence similarities
      Belongs to the lin-7 family.
      Contains 1 L27 domain.
      Contains 1 PDZ (DHR) domain.
    • Domain
      The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.
      The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.
      The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes.
    • Cellular localization
      Cell membrane. Basolateral cell membrane. Cell junction. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > tight junction. Cell junction > synapse > synaptosome. Enriched in synaptosomes and at epithelial cell-cell junctions (By similarity). Mainly basolateral in renal epithelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • hLin-7 antibody
      • Lin 7 homolog A (C. elegans) antibody
      • LIN 7A antibody
      • Lin-7A antibody
      • LIN7 antibody
      • LIN7A antibody
      • LIN7A_HUMAN antibody
      • MALS-1 antibody
      • Mammalian LIN 7 1 antibody
      • Mammalian lin-seven protein 1 antibody
      • Protein lin-7 homolog A antibody
      • Tax interaction protein 33 antibody
      • TIP-33 antibody
      • Veli-1 antibody
      • VELI1 antibody
      • Vertebrate lin-7 homolog 1 antibody
      • Vertebrate LIN7 homolog 1 antibody
      see all

    Images

    • All lanes : Anti-LIN7A antibody [EPR12609] (ab174297) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Mouse brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 26 kDa

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab174297 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Luo C  et al. microRNA-501-3p suppresses metastasis and progression of hepatocellular carcinoma through targeting LIN7A. Cell Death Dis 9:535 (2018). Read more (PubMed: 29749382) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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