Key features and details
- Chicken polyclonal to LINE-1 ORF2p
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
Product nameAnti-LINE-1 ORF2p antibody
DescriptionChicken polyclonal to LINE-1 ORF2p
The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein.
Database link: O00370
- induced bacterially expressed human ORF2
Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening product.
ab106004 is intended for use in studying control of L1 retrotransposons.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
Purification notesab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer.
Our Abpromise guarantee covers the use of ab106004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 149 kDa.|
- SwissProt: O00370 Human
- L1/ORF2 antibody
- LINE 1 ORF2p antibody
- LINE-1 retrotransposable element ORF2 protein antibody
All lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution
Lane 1 : induced bacterially expressed human ORF2
Lane 2 : uninduced bacterially expressed human ORF2
Predicted band size: 149 kDa
Note: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human LINE-1 ORF2p endonuclease domain fusion protein.
ab106004 has been referenced in 2 publications.
- Romero MA et al. Acute and chronic resistance training downregulates select LINE-1 retrotransposon activity markers in human skeletal muscle. Am J Physiol Cell Physiol 314:C379-C388 (2018). PubMed: 29351416
- Thomas CA et al. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation. Cell Stem Cell 21:319-331.e8 (2017). PubMed: 28803918