Overview

  • Product name

    Anti-LINE-1 ORF2p antibody
  • Description

    Chicken polyclonal to LINE-1 ORF2p
  • Host species

    Chicken
  • Specificity

    The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons.

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein.
    Database link: O00370

  • Positive control

    • induced bacterially expressed human ORF2
  • General notes

    Dilute only prior to immediate use.
    Expiration date is one (1) year from date of opening product. 

    ab106004 is intended for use in studying control of L1 retrotransposons.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    IgY fraction
  • Purification notes

    ab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
ELISA 1/10000.

Target

  • Database links

  • Alternative names

    • L1/ORF2 antibody
    • LINE 1 ORF2p antibody
    • LINE-1 retrotransposable element ORF2 protein antibody
    • LINE1 ORF2p antibody
    • ORF2p antibody
    see all

Images

  • All lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution

    Lane 1 : induced bacterially expressed human ORF2
    Lane 2 : uninduced bacterially expressed human ORF2

    Predicted band size: 149 kDa



    Note: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human LINE-1 ORF2p endonuclease domain fusion protein.

References

This product has been referenced in:

  • Thomas CA  et al. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation. Cell Stem Cell 21:319-331.e8 (2017). Read more (PubMed: 28803918) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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