Human Predicted to work with:
Synthetic peptide corresponding to Human LINE-1 retrotransposable element ORF2 protein conjugated to Keyhole Limpet Haemocyanin (KLH). Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. Database link: O00370
induced bacterially expressed human ORF2
Dilute only prior to immediate use. Expiration date is one (1) year from date of opening product.
ab106004 is intended for use in studying control of L1 retrotransposons.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
ab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer.
Western blot - Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004)
All lanes : Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004) at 1/2000 dilution
Lane 1 : induced bacterially expressed human ORF2 Lane 2 : uninduced bacterially expressed human ORF2
Predicted band size: 149 kDa
Note: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human L1/ORF2 endonuclease domain fusion protein.