For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Rabbit monoclonal [EPR9635] to LINGO3
, Flow Cytmore detailsUnsuitable for:
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human LINGO3 (UniProt P0C6S8).
- SH-SY5Y, U87-MG, 293T and NCI-H460 cell lysates; 293T cells; SH-SY5Y cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
||1/250 - 1/500.
||1/10 - 1/100.
||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Information by UniProt
- hCG2040376 antibody
- LERN2 antibody
- Leucine rich repeat and Ig domain containing 3 antibody
Western blot - Anti-LINGO3 antibody [EPR9635] (ab169772)
All lanes : Anti-LINGO3 antibody [EPR9635] (ab169772) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : 293T cell lysate
Lane 4 : NCI-H460 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LINGO3 antibody [EPR9635] (ab169772)
Immunofluorescent analysis of 293T cells labeling LINGO3 with ab169772 at 1/250 dilution (green). DAPI nuclear staining (blue).
Flow Cytometry - Anti-LINGO3 antibody [EPR9635] (ab169772)
Flow cytometry analysis of permeabilized SH-SY5Y cells labeling LINGO3 with ab169772 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Immunoprecipitation - Anti-LINGO3 antibody [EPR9635] (ab169772)
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab169772.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"