Overview

  • Product name
    Anti-LINGO3 antibody [EPR9635]
    See all LINGO3 primary antibodies
  • Description
    Rabbit monoclonal [EPR9635] to LINGO3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human LINGO3 (UniProt P0C6S8).

  • Positive control
    • SH-SY5Y, U87-MG, 293T and NCI-H460 cell lysates; 293T cells; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9635
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    Images

    • All lanes : Anti-LINGO3 antibody [EPR9635] (ab169772) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : NCI-H460 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 65 kDa

    • Immunofluorescent analysis of 293T cells labeling LINGO3 with ab169772 at 1/250 dilution (green). DAPI nuclear staining (blue).
    • Flow cytometry analysis of permeabilized SH-SY5Y cells labeling LINGO3 with ab169772 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
    • Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab169772.

    References

    ab169772 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169772.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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