Recombinant Anti-LINGO3 antibody [EPR9635] - BSA and Azide free (ab249518)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9635] to LINGO3 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LINGO3 antibody [EPR9635] - BSA and Azide free
See all LINGO3 primary antibodies -
Description
Rabbit monoclonal [EPR9635] to LINGO3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IP, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249518 is the carrier-free version of ab169772.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9635 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 65 kDa. |
Target
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Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 645191 Human
- Entrez Gene: 237403 Mouse
- Entrez Gene: 690755 Rat
- Omim: 609792 Human
- SwissProt: P0C6S8 Human
- SwissProt: Q6GQU6 Mouse
- Unigene: 371587 Human
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Alternative names
- hCG2040376 antibody
- LERN2 antibody
- Leucine rich repeat and Ig domain containing 3 antibody
see all
Images
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All lanes : Anti-LINGO3 antibody [EPR9635] (ab169772) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : 293T cell lysate
Lane 4 : NCI-H460 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDaThis data was developed using ab169772, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249518 has not yet been referenced specifically in any publications.