Recombinant Anti-LINGO4 antibody [EPR12600] - BSA and Azide free (ab249823)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12600] to LINGO4 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LINGO4 antibody [EPR12600] - BSA and Azide free
See all LINGO4 primary antibodies -
Description
Rabbit monoclonal [EPR12600] to LINGO4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human LINGO4. The exact sequence is proprietary.
Database link: Q6UY18 -
General notes
ab249823 is the carrier-free version of ab174832.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12600 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 64 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Relevance
The exact function of LINGO4 remains unknown. -
Cellular localization
Membrane; Single pass type I membrane protein -
Database links
- Entrez Gene: 339398 Human
- Entrez Gene: 320747 Mouse
- Entrez Gene: 499668 Rat
- Omim: 609794 Human
- SwissProt: Q6UY18 Human
- SwissProt: Q149C3 Mouse
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Alternative names
- DAAT9248 antibody
- Leucine rich repeat and Ig domain containing 4 antibody
- Leucine rich repeat and immunoglobulin like domain containing nogo receptor interacting protein 4 antibody
see all
Images
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All lanes : Anti-LINGO4 antibody [EPR12600] (ab174832) at 1/10000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 64 kDaThis data was developed using ab174832, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249823 has not yet been referenced specifically in any publications.