Overview

  • Product name

    Anti-LIP1 antibody
  • Description

    Rabbit polyclonal to LIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human LIP1 aa 900-950 (internal sequence).
    Database link: NP_443134.2

  • Positive control

  • General notes

     This product was previously labelled as STK11IP

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 121 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May regulate STK11 function by controlling its subcellular localization.
  • Sequence similarities

    Belongs to the STK11IP family.
    Contains 8 LRR (leucine-rich) repeats.
  • Cellular localization

    Cytoplasm. Some cells show granular or punctuate expression. Colocalizes with STK11 and SMAD4 in granular or punctuate structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1898 antibody
    • LIP1 antibody
    • LKB1 interacting protein 1 antibody
    • LKB1 interacting protein antibody
    • LKB1-interacting protein 1 antibody
    • LKB1IP antibody
    • S11IP_HUMAN antibody
    • Serine/threonine kinase 11 interacting protein antibody
    • Serine/threonine-protein kinase 11-interacting protein antibody
    • STK11 interacting protein antibody
    • stk11ip antibody
    • STK11IP1 antibody
    see all

Images

  • All lanes : Anti-LIP1 antibody (ab90573) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 121 kDa


    Exposure time: 30 seconds
  • ab90573 at 1 µg/ml detecting LIP1 following immunoprecipitation.
    Lane 1; IP using ab90573
    Lane 2; IP using an antibody to an upstream epitope of LIP1 
    Lane 3; IP using control IgG
    All antibodies used at 3 &microg/mg lysate. 1 mg of lysate precipitated and 20% loaded.
    Developed using the ECL technique with a 30 second exposure.

References

ab90573 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Protein A
Sample
Human Cell lysate - whole cell (Breast)
Specification
Breast
Total protein in input
1e+006 cells

Abcam user community

Verified customer

Submitted Dec 27 2013

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (Breast)
Specification
Breast
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Dec 24 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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