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Lipase Activity Assay Kit (Colorimetric) (ab102524) is a product where lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (OD=570nm).
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This assay is rapid, simple, sensitive, and reliable for activity screening of lipase. This kit detects lipase activity as low as 0.02mU per well.
For HTP analysis, we recommend Lipase Activity Assay Kit II (Colorimetric) (ab102525) or Lipase Activity Assay Kit III (Fluorometric) (ab118969).
|Assay Buffer||WM||1 x 25ml|
|Enzyme Mix (lyophilized)||Green||1 vial|
|Glycerol Standard||Yellow||1 x 0.2ml|
|Lipase positive control||Purple||1 vial|
|Lipase Substrate||Blue||1 vial|
|OxiRed Probe||Red||1 x 0.2ml|
Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"