Lipase Assay Kit II (Colorimetric) (ab102525)

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Overview

  • Product name

    Lipase Assay Kit II (Colorimetric)
    See all Lipase kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts

  • Assay type

    Enzyme activity

  • Assay time

    1h 30m

  • Product overview

    Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.


    In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (Absorbance=412 nm). This kit is suitable for high throughput analysis (HTP).


    Lipase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with a microplate reader at 3 min and at between 60 and 90 min

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - Lipase Detection Kit II (ab102525)
    Functional Studies - Lipase Detection Kit II (ab102525)
    Lipase Detection in a Sample and Positive Control
  • Functional Studies - Lipase Detection Kit II (ab102525)
    Functional Studies - Lipase Detection Kit II (ab102525)
    TNB Standard Curve

Protocols

References

ab102525 has not yet been referenced specifically in any publications.

Publishing research using ab102525? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hello,

I have a question regarding your Lipase Detection Kit II (product code ab102525) and Lipase Detection Kit III (ab118969). In order to determine if these products might be potentially useful for our application, I would need to know more about the structures of the substrates.

I understand that you can probably not release full detailed information regarding the chemical structure. I would just like to know if the substrates are triglyceride derivatives, or if they are more simple esters with only one hydrophobic fatty acid like chain.

I look forward to hearing from you.

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Answer

Thank you for contacting Abcam.

The substates for ab102525 and ab118969 are triglyceride derivatives.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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