Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Product nameLipase Assay Kit II (Colorimetric)
See all Lipase kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay typeEnzyme activity
Assay time1h 30m
Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.
In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (Absorbance=412 nm). This kit is suitable for high throughput analysis (HTP).
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader at 3 min and at between 60 and 90 min
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests DTNB Probe Red 1 vial Lipase AssayBuffer WM 1 x 25ml Lipase Positive Control Purple 1 vial Lipase Substrate Blue 1 x 500µl TNB Standard (5 mM) Amber 1 vial
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
RelevanceLipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
ab102525 has not yet been referenced specifically in any publications.