Product nameLipase Assay Kit III (Fluorometric)
See all Lipase kits
Sample typeCell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
Assay typeEnzyme activity
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm. This kit can be used to detect Lipase as low as 0.1 µU/well.
The lipase assay kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput assay of Lipase activity.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyse with a microplate reader immediately and at 30-60 min
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Lipase Assay Buffer WM 1 x 25ml Lipase Positive Control (lyophilized) Purple 1 vial Lipase Substrate Red 1 x 200µl Methylresorufin Standard (0.1 mM) Yellow 1 x 40µl
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
RelevanceLipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
ab118969 has not yet been referenced specifically in any publications.