The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Involvement in disease
Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency) [MIM:151670].
Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.