Anti-LIPC antibody (ab217360)
Key features and details
- Rabbit polyclonal to LIPC
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIPC antibody
See all LIPC primary antibodies -
Description
Rabbit polyclonal to LIPC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human LIPC aa 335-360 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:KSKRLFLVTRAQSPFKVYHYQFKIQF
Database link: P11150 -
Positive control
- HeLa cell lysate; Human liver carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab217360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/100 - 1/1000. Predicted molecular weight: 56 kDa. |
Target
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Function
Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. -
Involvement in disease
Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency) [MIM:151670]. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3990 Human
- Entrez Gene: 15450 Mouse
- Entrez Gene: 24538 Rat
- Omim: 151670 Human
- SwissProt: P11150 Human
- SwissProt: P27656 Mouse
- SwissProt: P07867 Rat
- Unigene: 654472 Human
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Alternative names
- HDLCQ12 antibody
- Hepatic lipase antibody
- Hepatic triacylglycerol lipase antibody
see all
Images
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Western blot - Anti-LIPC antibody (ab217360)Anti-LIPC antibody (ab217360) at 1/300 dilution + HeLa cell lysate
Secondary
Goat Anti-Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 56 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIPC antibody (ab217360)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded Human liver carcinoma tissue labeling LIPC with ab217360 at 1/200 dilution overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Antigen retrieval by boiling in sodium citrate buffer (pH 6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37°C for 20 minutes.
Protocols
Datasheets and documents
References (0)
ab217360 has not yet been referenced specifically in any publications.
