Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine liver cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
murine liver cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 1xTST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Peroxidase labeled anti-rabbit IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 58 kDa Non-specific: 75 kDa
Positive control
human liver cellls (HepG2)
Sabrina Hütter
Verified customer
Submitted Nov 14 2018