Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5711(B)] to LIPF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Product nameAnti-LIPF antibody [EPR5711(B)]
See all LIPF primary antibodies
DescriptionRabbit monoclonal [EPR5711(B)] to LIPF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human LIPF aa 350-450 (C terminal). The exact sequence is proprietary.
- Human stomach and Mouse small intestine lysates; Human liver tissue.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).|
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
- Information by UniProt
- Gastric lipase antibody
- Gastric triacylglycerol lipase antibody
- gL antibody
All lanes : Anti-LIPF antibody [EPR5711(B)] (ab124831) at 1/1000 dilution
Lane 1 : Human stomach lysate
Lane 2 : Mouse small intestine lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
ab124831, at 1/250 dilution, staining LIPF in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab124831 has not yet been referenced specifically in any publications.