Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP12065] to LIPH
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-LIPH antibody [EP12065]
See all LIPH primary antibodies
DescriptionRabbit monoclonal [EP12065] to LIPH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human LIPH aa 350-450. The exact sequence is proprietary.
Database link: Q8WWY8
- Human prostate cancer and Human pancreas lysates; Human glioma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab192615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/20000. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionHydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).
Tissue specificityPresent in intestine (at protein level). Expressed in colon, prostate, kidney, pancreas, ovary, testis, intestine, lung and pancreas. Expressed at lower level in brain, spleen and heart. In hair, it is prominently expressed in hair follicles, including the stem cell-rich bulge region.
Involvement in diseaseDefects in LIPH are the cause of hypotrichosis localized autosomal recessive type 2 (LAH2) [MIM:604379]; also known as alopecia universalis congenita Mari type. A condition characterized by the presence of less than the normal amount of hair. Affected individuals have sparse or absent scalp, axillary and body hair and sparse eyebrows and eyelashes.
Defects in LIPH are the cause of woolly hair autosomal recessive type 2 (ARWH2) [MIM:604379]. A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrexis nodosa and tapered ends. Some individuals may present with hypotrichosis.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
Cellular localizationSecreted. Membrane.
- Information by UniProt
- AH antibody
- ARWH2 antibody
- LAH2 antibody
Anti-LIPH antibody [EP12065] (ab192615) at 1/20000 dilution + Human prostate cancer tissue lysate at 10 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling LIPH with ab192615 at 1/1000 dilution (1μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
All lanes : Anti-LIPH antibody [EP12065] (ab192615) at 1/5000 dilution
Lane 1 : Human pancreas tissue lysate
Lane 2 : Human prostate cancer tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
ab192615 has been referenced in 1 publication.
- Li Y et al. Lipase member H is a downstream molecular target of hypoxia inducible factor-1a and promotes papillary thyroid carcinoma cell migration in BCPAP and KTC-1 cell lines. Cancer Manag Res 11:931-941 (2019). PubMed: 30774423