Overview

  • Product name

    Anti-LIPH antibody [EP12065]
    See all LIPH primary antibodies
  • Description

    Rabbit monoclonal [EP12065] to LIPH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LIPH aa 350-450. The exact sequence is proprietary.
    Database link: Q8WWY8

  • Positive control

    • Human prostate cancer and Human pancreas lysates; Human glioma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).
  • Tissue specificity

    Present in intestine (at protein level). Expressed in colon, prostate, kidney, pancreas, ovary, testis, intestine, lung and pancreas. Expressed at lower level in brain, spleen and heart. In hair, it is prominently expressed in hair follicles, including the stem cell-rich bulge region.
  • Involvement in disease

    Defects in LIPH are the cause of hypotrichosis localized autosomal recessive type 2 (LAH2) [MIM:604379]; also known as alopecia universalis congenita Mari type. A condition characterized by the presence of less than the normal amount of hair. Affected individuals have sparse or absent scalp, axillary and body hair and sparse eyebrows and eyelashes.
    Defects in LIPH are the cause of woolly hair autosomal recessive type 2 (ARWH2) [MIM:604379]. A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrexis nodosa and tapered ends. Some individuals may present with hypotrichosis.
  • Sequence similarities

    Belongs to the AB hydrolase superfamily. Lipase family.
  • Cellular localization

    Secreted. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AH antibody
    • ARWH2 antibody
    • LAH2 antibody
    • Lipase H antibody
    • Lipase member H antibody
    • LIPH antibody
    • LIPH_HUMAN antibody
    • LPD lipase related protein antibody
    • LPD lipase-related protein antibody
    • LPDLR antibody
    • Membrane associated phosphatidic acid selective phospholipase A1 alpha antibody
    • Membrane bound phosphatidic acid selective phospholipase A1 antibody
    • Membrane-associated phosphatidic acid-selective phospholipase A1-alpha antibody
    • mPA PLA1 antibody
    • mPA-PLA1 alpha antibody
    • MPAPLA1 antibody
    • OTTHUMP00000210343 antibody
    • OTTHUMP00000210344 antibody
    • Phospholipase A1 member B antibody
    • PLA1B antibody
    see all

Images

  • Anti-LIPH antibody [EP12065] (ab192615) at 1/20000 dilution + Human prostate cancer tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa

  • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling LIPH with ab192615 at 1/1000 dilution (1μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-LIPH antibody [EP12065] (ab192615) at 1/5000 dilution

    Lane 1 : Human pancreas tissue lysate
    Lane 2 : Human prostate cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa

References

This product has been referenced in:

  • Li Y  et al. Lipase member H is a downstream molecular target of hypoxia inducible factor-1a and promotes papillary thyroid carcinoma cell migration in BCPAP and KTC-1 cell lines. Cancer Manag Res 11:931-941 (2019). Read more (PubMed: 30774423) »
See 1 Publication for this product

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