Anti-Lipid A antibody [26-5] (ab8467)
Key features and details
- Mouse monoclonal [26-5] to Lipid A
- Suitable for: Agglutination, ELISA
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-Lipid A antibody [26-5] -
Description
Mouse monoclonal [26-5] to Lipid A -
Host species
Mouse -
Tested applications
Suitable for: Agglutination, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Lipid A. Synthetic Lipid A.
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General notes
Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 99.88% PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
This product is sterile filtered. -
Primary antibody notes
Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria. -
Clonality
Monoclonal -
Clone number
26-5 -
Myeloma
unknown -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Agglutination |
Use at an assay dependent concentration.
Use NEAT. |
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ELISA |
1/10.
in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density. |
Notes |
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Agglutination
Use at an assay dependent concentration. Use NEAT. |
ELISA
1/10. in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density. |
Target
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Relevance
Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction. -
Cellular localization
Secreted
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab8467 has been referenced in 5 publications.
- Kameli N et al. Characterization of Feces-Derived Bacterial Membrane Vesicles and the Impact of Their Origin on the Inflammatory Response. Front Cell Infect Microbiol 11:667987 (2021). PubMed: 34026664
- Schlichtner S et al. Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis. Int Immunopharmacol 100:108155 (2021). PubMed: 34543981
- Vargas-Caraveo A et al. Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions. Sci Rep 7:13113 (2017). PubMed: 29030613
- Martin I et al. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol 194:3924-36 (2015). PubMed: 25780044
- Kadowaki T et al. Functional Characterization of Lipopolysaccharide derived from Symbiotic Bacteria in Rice as a Macrophage-activating Substance. Anticancer Res 31:2467-76 (2011). WB . PubMed: 21873161