Overview

  • Product name
    Anti-Lipid A antibody [26-5]
    See all Lipid A primary antibodies
  • Description
    Mouse monoclonal [26-5] to Lipid A
  • Host species
    Mouse
  • Tested applications
    Suitable for: Agglutination, ELISA, WBmore details
  • Immunogen

    Synthetic Lipid A.

  • General notes


    Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is sterile filtered.
  • Primary antibody notes
    Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.
  • Clonality
    Monoclonal
  • Clone number
    26-5
  • Myeloma
    unknown
  • Isotype
    IgG2b
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Agglutination Use at an assay dependent concentration. Use NEAT.
ELISA 1/10.

in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.

WB Use at an assay dependent concentration. PubMed: 21873161

Target

  • Relevance
    Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
  • Cellular localization
    Secreted

References

This product has been referenced in:
  • Vargas-Caraveo A  et al. Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions. Sci Rep 7:13113 (2017). Read more (PubMed: 29030613) »
  • Martin I  et al. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol 194:3924-36 (2015). Read more (PubMed: 25780044) »
See all 3 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Answer

We do not have in-house data demonstrating relative specificity of antibody ab8467 for lipid A versus IVa. The following reference discusses this antibody, clone 26-5, but I do not know if it will help answer your question, as we do not have access to the article, just the abstract.

Binding characteristics and cross-reactivity of three different antilipid A monoclonal antibodies. J Immunol. 1989 Dec 15;143(12):4053-60.

Read More

Answer

Thank you for contacting us.
This antibody is supplied at a concentration of 0.2 mg/mL and each vial contains 200ug of antibody. For ELISA, we recommend using this antibody at a dilution of 1:10 for ELISA and 1:200 for WB. These dilutions will need to be further optimized based on your specific sample types and protocol.

I hope this helps, please let me know if you need any additional information.

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Answer

Thank you for your enquiry. Unfortunately, we do not know which region this product will detect but we know that it reacts with the hydrophilic part of Lipid-A. I am afraid that the peptide sequence has not been mapped.

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Answer

There is no specific reason for using tapwater. You can also use Milli-Q, but we did not test this antibody on Western blot.

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Answer

Thank you for your enquiry. Unfortunately, the only anti-lipid A antibody which we currently carry is ab8467. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have any more questions, please contact us again.

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Answer

Due to a recent customer enquiry our understanding now is that this antibody reacts with the hydrophilic region of synthetic lipid A (with no sugars attached) and not with Re-LPS (which add KDO sugars to the hydrophilic region of lipid A and block the antibody antigen interaction. This is why the datasheet was changed.

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Answer

Unfortunately, we do not have any record of this Lipopolysaccharide antibody being tested on the E.Coli K-12 strain.

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Question
Answer

Just to follow on from our phone call. I would expect this antibody to work in IC as it works in rough bacterial preparations, although it has only been tested in ELISA. However, we have a policy of not providing samples sizes, as we find it takes up too much of our time to be worthwhile.

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Question
Answer

The immunogen for ab8467 was the Re mutant of Salmonella typhimurium. The antibody recognises the lipid A/KDO region of LPS

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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