Key features and details
- Goat polyclonal to Lipid A
- Suitable for: ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Lipid A antibody
See all Lipid A primary antibodies
DescriptionGoat polyclonal to Lipid A
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Species independent
Whole cells prep of Lipid A from E. coli O157.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notesSodium sulfate precipitation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab20001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Fix with acetone.|
RelevanceLipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
ab20001 has been referenced in 4 publications.
- Pedersen GA et al. The basolateral vesicle sorting machinery and basolateral proteins are recruited to the site of enteropathogenic E. coli microcolony growth at the apical membrane. PLoS One 12:e0179122 (2017). PubMed: 28636623
- Jensen HH et al. Tir Is Essential for the Recruitment of Tks5 to Enteropathogenic Escherichia coli Pedestals. PLoS One 10:e0141871 (2015). ICC/IF . PubMed: 26536015
- Shifrin DA et al. Dynamics of brush border remodeling induced by enteropathogenic E. coli. Gut Microbes 5:504-16 (2014). PubMed: 25076126
- Miyata ST et al. Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun 79:2941-9 (2011). PubMed: 21555399