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Lipid Assay Kit (neutral lipids) (ab242307)

  • Datasheet
  • Protocol Booklet
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Lipid Quantification Kit (Fluorometric) standard curve in methanol/chloroform.
  • Detection of triolein or lysophosphatidylcholine
  • Lipid Quantification Kit (Fluorometric) standard curve in water.
  • Detection of lipids in aqueous samples.

Key features and details

  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Cell culture supernatant, Plasma, Serum, Tissue Culture Media
  • Sensitivity: 4000 µg/dl

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Overview

  • Product name

    Lipid Assay Kit (neutral lipids)
    See all Lipids kits
  • Detection method

    Fluorescent
  • Sample type

    Cell culture supernatant, Serum, Plasma, Tissue Culture Media
  • Sensitivity

    4000 µg/dl
  • Product overview

    Lipids are a diverse group of molecules that include monoglycerides, diglycerides, triglycerides, fats, sterols, and others. Not only do lipids define and preserve cellular membrane integrity, but they are also involved in cellular processes such as membrane trafficking, signal transduction, apoptosis, and energy storage. Perturbation in the metabolism of lipids has been linked to many diseases such as cancer, diabetes, Alzheimer’s disease, and coronary heart disease. In order to study lipids, they must often be extracted first from tissues or cellular cultures and then quantified. Some methods for lipid quantification have been described that are quite sensitive, however they require expensive equipment such as an HPLC machine, light scattering detection technology, or a latroscan TLC-FID analyzer.


    ab242307 specifically measures the neutral lipid content of samples using a lipid binding molecule that fluoresces brightly only when bound to lipids, resulting in a simple fluorescence readout. First, a crude or purified lipid source is applied to a 96-well plate. Then organic solvents are removed, followed by the addition of the fluorescent reagent. Lipid binding to the fluorescent reagent results in an increase in fluorescence that is easily detected on a microplate reader. This kit has a detection sensitivity of 3.9 mg/dL and is at least 10 times more sensitive than the Vanillin method. Each kit provides sufficient reagents to perform 100 assays including standards and unknown samples.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 100 tests
    Fluorometric Reagent (100X) 1 x 200µl
    Lipid Standard 1 x 100µl

Associated products

    Images

    • Lipid Quantification Kit (Fluorometric) standard curve in methanol/chloroform.
      Lipid Quantification Kit (Fluorometric) standard curve in methanol/chloroform.
    • Detection of triolein or lysophosphatidylcholine
      Detection of triolein or lysophosphatidylcholine

      Purified triolein (blue bars) or lysophosphatidylcholine (green bars) were diluted in Methanol/Chloroform and analyzed using the Lipid Quantification Kit (Fluorometric).

    • Lipid Quantification Kit (Fluorometric) standard curve in water.
      Lipid Quantification Kit (Fluorometric) standard curve in water.
    • Detection of lipids in aqueous samples.
      Detection of lipids in aqueous samples.

      Undiluted human serum, human plasma, and water were analyzed using the Lipid Quantification Kit (Fluorometric).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab242307? Please let us know so that we can cite the reference in this datasheet.

    ab242307 has not yet been referenced specifically in any publications.

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