Lipid Assay Kit (unsaturated fatty acids) (ab242305)
Key features and details
- Detection method: Colorimetric
- Sample type: Cell culture media, Plasma, Serum, Tissue
Overview
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Product name
Lipid Assay Kit (unsaturated fatty acids)
See all Lipids kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue, Cell culture media -
Product overview
The Lipid Assay Kit (unsaturated fatty acids) (ab242305) measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method resulting in a simple colorimetric readout amenable to multi-well plate detection.
First, a crude or purified lipid source is applied to a 96 well plate. Then concentrated sulfuric acid is added and the samples are heated to solubilize and prime the total lipid sample, followed by the addition of vanillin in an acid solution. The lipids react with vanillin in the presence of the acids to form a colorimetric product that is easily detected on a microplate reader.
Each kit provides sufficient reagents to perform 100 assays including standards and unknown samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Purified Lipid Standard 1 x 100µl Vanillin Reagent 1 x 10ml
Images
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Lipid Quantification Kit Standard Curve.
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Detection of lipids from Human Serum or Human Plasma.Fifteen microliters of undiluted Human Serum, Human Plasma, or Negative Control buffer were analyzed using ab242305.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab242305 has been referenced in 2 publications.
- Qi G et al. ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism. Cell Rep 34:108572 (2021). PubMed: 33406436
- Rivas Serna IM et al. A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 33182805