Lipid Peroxidation (4-HNE) Assay Kit (ab238538)
Key features and details
- Assay type: Competitive
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Purified protein, Serum, Tissue Lysate
Overview
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Product name
Lipid Peroxidation (4-HNE) Assay Kit -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Competitive -
Species reactivity
Reacts with: Vertebrata -
Product overview
Lipid Peroxidation (4-HNE) Assay Kit (ab238538) is designed for the rapid detection and quantitation of 4-hydroxynonenal (4-HNE)-protein adducts.
4-HNE is a well known by-product of lipid peroxidation and is widely accepted as a stable marker for oxidative stress. This Competitive ELISA kit will allow the quantitation of 4-HNE adduct in protein samples determined by comparing its absorbance with that of a known 4-HNE-BSA standard curve.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 32 tests 100X Conjugate Diluent 1 x 300µl 1 x 100µl 10X Wash Buffer 1 x 100ml 1 x 30ml Protein Binding Strip Well Plate 1 unit 1 unit Anti-HNE Antibody (1000X) 1 x 10µl 1 x 5µl Assay Diluent 1 x 50ml 1 x 20ml HNE Conjugate 1 x 50µl 1 x 20µl HNE-BSA Standard 1 x 250µl 1 x 100µl Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl 1 x 20µl Stop Solution 1 x 12ml 1 x 4ml Substrate Solution 1 x 12ml 1 x 4ml -
Research areas
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Relevance
Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress. -
Cellular localization
Cytoplasmic -
Alternative names
- 4 HNE
- 4-Hydroxy-2-Nonenal
Images
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab238538 has been referenced in 33 publications.
- Chuang JZ et al. Retinal pigment epithelium-specific CLIC4 mutant is a mouse model of dry age-related macular degeneration. Nat Commun 13:374 (2022). PubMed: 35042858
- Ammar OA et al. Melatonin is a potential oncostatic agent to inhibit HepG2 cell proliferation through multiple pathways. Heliyon 8:e08837 (2022). PubMed: 35141433
- Ansermet C et al. Renal tubular peroxisomes are dispensable for normal kidney function. JCI Insight 7:N/A (2022). PubMed: 35191396
- Mikes M et al. Translating PUFA omega 6:3 ratios from wild to captive hibernators (Urocitellus parryii) enhances sex-dependent mass-gain without increasing physiological stress indicators. J Comp Physiol B 192:529-540 (2022). PubMed: 35503574
- Yuan L et al. Dihydroquercetin Attenuates Silica-Induced Pulmonary Fibrosis by Inhibiting Ferroptosis Signaling Pathway. Front Pharmacol 13:845600 (2022). PubMed: 35645837